![]() Sc pDNA with homogeneity higher than 90 and virtually free from RNA is obtained. Capto adhere is a multimodal anion exchange chromatography resin for intermediate purification and polishing of monoclonal antibodies after the Protein A capture step, giving the possibility to design a two-step chromatographic downstream process. 2-step elution with NaCl is used to remove open circular pDNA and RNA from sc pDNA. Select from over 300 resins to pack into OPUS Pre-packed Columns for process development. Together with a Protein A capture step, like MabSelect SuRe, Capto adhere allows the design of a two-step chromatographic process for mAbs.įor optimal performance of Capto adhere, screening and optimization of loading conditions is recommended, preferably using modern high-throughput process development tools, such as PreDictor 96-well filter plates. Supercoiled pDNA is purified by multimodal chromatography with Capto adhere resin. 4 pH range where resin can be operated without significant change in function. Second step in mAb chromatographic processĬapto adhere can remove key contaminants such as DNA, host cell proteins (HCP), leached protein A, dimers and larger aggregates, and viruses in a single step. The same trends were observed The Capto family showed the highest resistance to deformation (Capto Adhere- 2.7, Capto Q-1.92 1/min1) through to Sepharose CL-6B, Sepharose 4 Fast Flow and Sepharose CL-4B which exhibited the lowest slurry resistances (0.59, 0.4, 0.3 1/min-1respectively). The strong multimodal ligand gives a different selectivity compared to traditional ion exchange (IEX) resins. High capacity and productivity in flow-through mode.ĭifferent selectivity from traditional IEX resinsĬapto adhere is composed of a rigid, high-flow agarose matrix and a novel multimodal ligand. A multimodal anion exchange resin designed for post-protein A purification of monoclonal antibodies at process scale. Capto adhere is a multimodal BioProcess medium (resin) designed for post-protein A purification of monoclonal antibodies (MAbs) at process scale (Fig 1).Removes contaminants to formulation levels.Two-step chromatographic process with protein A affinity, saves time and cuts operating costs. Cytiva Capto Adhere ImpRes, 100mL, 40um Particle Size, 45 to 85mg Mab mL Binding.Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.Wide operational window of pH and conductivity.A multimodal anion exchange resin designed for post-protein A purification of monoclonal antibodies at process scale. Capto adhere ImpRes is a BioProcess chromatography medium (resin) for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules.
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